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hg-u133a cdna microarray chips  (Thermo Fisher)


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    Thermo Fisher hg-u133a cdna microarray chips
    Hg U133a Cdna Microarray Chips, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdna+chip+microarray/pmc04824563-129-14-18
    Average 90 stars, based on 1 article reviews
    hg-u133a cdna microarray chips - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Microarray:

    Article Title: Microfluidic reaction vessel array with patterned films
    Article Snippet: .. Early biochips were based on the idea of a DNA microarray, e.g., the GeneChip DNA array from Affymetrix, which is a piece of glass, plastic or silicon substrate on which DNA molecules (probes) are affixed in an array. ..

    Article Title: Leveraging Large-Scale Biobanks for Therapeutic Target Discovery.
    Article Snippet: .. Genotyping of ARIC samples was performed on the Affymetrix 6.0 DNA microarray and imputed to the TOPMed reference panel (Freeze 5b). ..

    Article Title: High-throughput engineered tumor organoids reveal ROCK signaling as an immunotherapeutic target in triple-negative breast cancer.
    Article Snippet: .. TNBC (IHC) subtypes prognostic analysis Prognostic analysis of TNBC (IHC) subtypes and gene expression studies were performed using bc-GenExMiner v5.2,47 which integrates TCGA, SCAN-B RNA-seq, and Affymetrix®, METABRIC DNA microarray datasets. ..

    Article Title: Interleukin-2 receptor γ blocks the development of Leydig cells from stem/progenitor cells in male rats via JAK1-STAT3 pathway and GSDMD-mediated pyroptosis.
    Article Snippet: Interleukin-2 (IL-2) receptor γ (IL2RG) is present in the Leydig cell lineage, but its functional role remains elusive.. To investigate how IL2RG impacts Leydig cell development from stem cells and elucidate the underlying mechanisms, we used a rat model of ethane dimethanesulfonate (EDS)-induced cell depletion in Wild-type (WT) and Il2rg knockout (KO) mice.. WT and KO male rats received intratesticular IL-2 injections (1 and 10 ng/testis) from day 7 to day 21 post-EDS.

    Article Title: Leveraging and partitioning polygenic risk scores to identify cancer-related proteins
    Article Snippet: .. Genotyping of ARIC samples was performed on the Affymetrix 6.0 DNA microarray and imputed to the TOPMed reference panel (Freeze 5b). ..

    Article Title: Empowering chemotherapy-induced antitumor immunity by multi-targeted synergistic combination nanomedicine for triple-negative breast cancer
    Article Snippet: .. The gene expression profiles are generated through standardized DNA microarray analysis procedures, which involve RNA extraction from tumor samples, reverse transcription to cDNA, hybridization to oligonucleotide microarray chips (e.g., Affymetrix U133A or U133 Plus 2.0), followed by fluorescence-based signal detection and normalization using algorithms such as RMA or MAS5 [ ]. ..

    Article Title: Deoxy-cytidine or uridine derivatives for use in cancer therapies
    Article Snippet: .. Microarray Method A comprises the following steps: 1) Extracting RNA from cancer/tumour cells of interest, said extraction preferably being performed using a Norgen Total RNA Purification kit (Norgen Biotek Cat nr. 17200); 2) Preparing Poly(A)+ RNA from the extracted RNA, preferably using a MEGApure kit according to the manufacturer's instructions (Ambion); 3) Preparing complementary DNA (cDNA) from the Poly(A)+ RNA by treatment with reverse transcriptase, i.e. performing reverse transcription; 4) Fragmenting the cDNA using TdT (terminal deoxynucleotidyl transferase); 5) Biotinylating the cDNA fragments using the GeneChip WT Terminal labelling kit (Affymetrix); 6 Repeating steps 1 to 5 with a reference cell line, wherein said reference cell line is MDA-MB-231; 7) Hybridizing 5.5 μg of the biotinylated cDNA fragments obtained in step 5 to a first DNA microarray at 45° C. for 16 hours, and hybridizing 5.5 μg of the biotinylated cDNA fragments obtained in step 6, to a DNA microarray at 45° C. for 16 hours, preferably wherein said microarrays are Affymetrix GeneChip Human Gene 2.0 ST Arrays (Applied Biosystems); 8) Washing and staining the hybridized microarrays, preferably in the Affymetrix GeneChip Fluidics Station 450 (Applied Biosystems); 9) Scanning said stained microarrays with the Affymetrix GeneChip Scanner 3000 7G utilising the Affymetrix GeneChip Command Console® software to produce raw data signal values in the form of CEL files; 10) Normalizing the CEL files to produce gene-level expression values using the implementation of the Robust Multiarray Average (RMA) in the Affymetrix software package (version 1.36.1), preferably as described in R. A. Irizarry et al., Exploration, normalization, and summaries of high density oligonucleotide array probe level data. ..

    Article Title: Enhanced Risk Prediction for Coronary Heart Disease by Leveraging Polygenic Risk Score and Clinical Risk Score in European Hypertensive Adults.
    Article Snippet: .. SNP genotype data of all participants were acquired from the Affymetrix 6.0 DNA microarray platform (Affymetrix, Santa Clara, CA, USA) and analyzed using the Birdseed variant-calling algorithm. ..

    DNA Array:

    Article Title: Microfluidic reaction vessel array with patterned films
    Article Snippet: .. Early biochips were based on the idea of a DNA microarray, e.g., the GeneChip DNA array from Affymetrix, which is a piece of glass, plastic or silicon substrate on which DNA molecules (probes) are affixed in an array. ..

    Immunohistochemistry:

    Article Title: High-throughput engineered tumor organoids reveal ROCK signaling as an immunotherapeutic target in triple-negative breast cancer.
    Article Snippet: .. TNBC (IHC) subtypes prognostic analysis Prognostic analysis of TNBC (IHC) subtypes and gene expression studies were performed using bc-GenExMiner v5.2,47 which integrates TCGA, SCAN-B RNA-seq, and Affymetrix®, METABRIC DNA microarray datasets. ..

    Gene Expression:

    Article Title: High-throughput engineered tumor organoids reveal ROCK signaling as an immunotherapeutic target in triple-negative breast cancer.
    Article Snippet: .. TNBC (IHC) subtypes prognostic analysis Prognostic analysis of TNBC (IHC) subtypes and gene expression studies were performed using bc-GenExMiner v5.2,47 which integrates TCGA, SCAN-B RNA-seq, and Affymetrix®, METABRIC DNA microarray datasets. ..

    Article Title: Empowering chemotherapy-induced antitumor immunity by multi-targeted synergistic combination nanomedicine for triple-negative breast cancer
    Article Snippet: .. The gene expression profiles are generated through standardized DNA microarray analysis procedures, which involve RNA extraction from tumor samples, reverse transcription to cDNA, hybridization to oligonucleotide microarray chips (e.g., Affymetrix U133A or U133 Plus 2.0), followed by fluorescence-based signal detection and normalization using algorithms such as RMA or MAS5 [ ]. ..

    RNA Sequencing:

    Article Title: High-throughput engineered tumor organoids reveal ROCK signaling as an immunotherapeutic target in triple-negative breast cancer.
    Article Snippet: .. TNBC (IHC) subtypes prognostic analysis Prognostic analysis of TNBC (IHC) subtypes and gene expression studies were performed using bc-GenExMiner v5.2,47 which integrates TCGA, SCAN-B RNA-seq, and Affymetrix®, METABRIC DNA microarray datasets. ..

    Expressing:

    Article Title: Interleukin-2 receptor γ blocks the development of Leydig cells from stem/progenitor cells in male rats via JAK1-STAT3 pathway and GSDMD-mediated pyroptosis.
    Article Snippet: Interleukin-2 (IL-2) receptor γ (IL2RG) is present in the Leydig cell lineage, but its functional role remains elusive.. To investigate how IL2RG impacts Leydig cell development from stem cells and elucidate the underlying mechanisms, we used a rat model of ethane dimethanesulfonate (EDS)-induced cell depletion in Wild-type (WT) and Il2rg knockout (KO) mice.. WT and KO male rats received intratesticular IL-2 injections (1 and 10 ng/testis) from day 7 to day 21 post-EDS.

    Article Title: Deoxy-cytidine or uridine derivatives for use in cancer therapies
    Article Snippet: .. Microarray Method A comprises the following steps: 1) Extracting RNA from cancer/tumour cells of interest, said extraction preferably being performed using a Norgen Total RNA Purification kit (Norgen Biotek Cat nr. 17200); 2) Preparing Poly(A)+ RNA from the extracted RNA, preferably using a MEGApure kit according to the manufacturer's instructions (Ambion); 3) Preparing complementary DNA (cDNA) from the Poly(A)+ RNA by treatment with reverse transcriptase, i.e. performing reverse transcription; 4) Fragmenting the cDNA using TdT (terminal deoxynucleotidyl transferase); 5) Biotinylating the cDNA fragments using the GeneChip WT Terminal labelling kit (Affymetrix); 6 Repeating steps 1 to 5 with a reference cell line, wherein said reference cell line is MDA-MB-231; 7) Hybridizing 5.5 μg of the biotinylated cDNA fragments obtained in step 5 to a first DNA microarray at 45° C. for 16 hours, and hybridizing 5.5 μg of the biotinylated cDNA fragments obtained in step 6, to a DNA microarray at 45° C. for 16 hours, preferably wherein said microarrays are Affymetrix GeneChip Human Gene 2.0 ST Arrays (Applied Biosystems); 8) Washing and staining the hybridized microarrays, preferably in the Affymetrix GeneChip Fluidics Station 450 (Applied Biosystems); 9) Scanning said stained microarrays with the Affymetrix GeneChip Scanner 3000 7G utilising the Affymetrix GeneChip Command Console® software to produce raw data signal values in the form of CEL files; 10) Normalizing the CEL files to produce gene-level expression values using the implementation of the Robust Multiarray Average (RMA) in the Affymetrix software package (version 1.36.1), preferably as described in R. A. Irizarry et al., Exploration, normalization, and summaries of high density oligonucleotide array probe level data. ..

    Generated:

    Article Title: Empowering chemotherapy-induced antitumor immunity by multi-targeted synergistic combination nanomedicine for triple-negative breast cancer
    Article Snippet: .. The gene expression profiles are generated through standardized DNA microarray analysis procedures, which involve RNA extraction from tumor samples, reverse transcription to cDNA, hybridization to oligonucleotide microarray chips (e.g., Affymetrix U133A or U133 Plus 2.0), followed by fluorescence-based signal detection and normalization using algorithms such as RMA or MAS5 [ ]. ..

    RNA Extraction:

    Article Title: Empowering chemotherapy-induced antitumor immunity by multi-targeted synergistic combination nanomedicine for triple-negative breast cancer
    Article Snippet: .. The gene expression profiles are generated through standardized DNA microarray analysis procedures, which involve RNA extraction from tumor samples, reverse transcription to cDNA, hybridization to oligonucleotide microarray chips (e.g., Affymetrix U133A or U133 Plus 2.0), followed by fluorescence-based signal detection and normalization using algorithms such as RMA or MAS5 [ ]. ..

    Reverse Transcription:

    Article Title: Empowering chemotherapy-induced antitumor immunity by multi-targeted synergistic combination nanomedicine for triple-negative breast cancer
    Article Snippet: .. The gene expression profiles are generated through standardized DNA microarray analysis procedures, which involve RNA extraction from tumor samples, reverse transcription to cDNA, hybridization to oligonucleotide microarray chips (e.g., Affymetrix U133A or U133 Plus 2.0), followed by fluorescence-based signal detection and normalization using algorithms such as RMA or MAS5 [ ]. ..

    Article Title: Deoxy-cytidine or uridine derivatives for use in cancer therapies
    Article Snippet: .. Microarray Method A comprises the following steps: 1) Extracting RNA from cancer/tumour cells of interest, said extraction preferably being performed using a Norgen Total RNA Purification kit (Norgen Biotek Cat nr. 17200); 2) Preparing Poly(A)+ RNA from the extracted RNA, preferably using a MEGApure kit according to the manufacturer's instructions (Ambion); 3) Preparing complementary DNA (cDNA) from the Poly(A)+ RNA by treatment with reverse transcriptase, i.e. performing reverse transcription; 4) Fragmenting the cDNA using TdT (terminal deoxynucleotidyl transferase); 5) Biotinylating the cDNA fragments using the GeneChip WT Terminal labelling kit (Affymetrix); 6 Repeating steps 1 to 5 with a reference cell line, wherein said reference cell line is MDA-MB-231; 7) Hybridizing 5.5 μg of the biotinylated cDNA fragments obtained in step 5 to a first DNA microarray at 45° C. for 16 hours, and hybridizing 5.5 μg of the biotinylated cDNA fragments obtained in step 6, to a DNA microarray at 45° C. for 16 hours, preferably wherein said microarrays are Affymetrix GeneChip Human Gene 2.0 ST Arrays (Applied Biosystems); 8) Washing and staining the hybridized microarrays, preferably in the Affymetrix GeneChip Fluidics Station 450 (Applied Biosystems); 9) Scanning said stained microarrays with the Affymetrix GeneChip Scanner 3000 7G utilising the Affymetrix GeneChip Command Console® software to produce raw data signal values in the form of CEL files; 10) Normalizing the CEL files to produce gene-level expression values using the implementation of the Robust Multiarray Average (RMA) in the Affymetrix software package (version 1.36.1), preferably as described in R. A. Irizarry et al., Exploration, normalization, and summaries of high density oligonucleotide array probe level data. ..

    Hybridization:

    Article Title: Empowering chemotherapy-induced antitumor immunity by multi-targeted synergistic combination nanomedicine for triple-negative breast cancer
    Article Snippet: .. The gene expression profiles are generated through standardized DNA microarray analysis procedures, which involve RNA extraction from tumor samples, reverse transcription to cDNA, hybridization to oligonucleotide microarray chips (e.g., Affymetrix U133A or U133 Plus 2.0), followed by fluorescence-based signal detection and normalization using algorithms such as RMA or MAS5 [ ]. ..

    Fluorescence:

    Article Title: Empowering chemotherapy-induced antitumor immunity by multi-targeted synergistic combination nanomedicine for triple-negative breast cancer
    Article Snippet: .. The gene expression profiles are generated through standardized DNA microarray analysis procedures, which involve RNA extraction from tumor samples, reverse transcription to cDNA, hybridization to oligonucleotide microarray chips (e.g., Affymetrix U133A or U133 Plus 2.0), followed by fluorescence-based signal detection and normalization using algorithms such as RMA or MAS5 [ ]. ..

    Extraction:

    Article Title: Deoxy-cytidine or uridine derivatives for use in cancer therapies
    Article Snippet: .. Microarray Method A comprises the following steps: 1) Extracting RNA from cancer/tumour cells of interest, said extraction preferably being performed using a Norgen Total RNA Purification kit (Norgen Biotek Cat nr. 17200); 2) Preparing Poly(A)+ RNA from the extracted RNA, preferably using a MEGApure kit according to the manufacturer's instructions (Ambion); 3) Preparing complementary DNA (cDNA) from the Poly(A)+ RNA by treatment with reverse transcriptase, i.e. performing reverse transcription; 4) Fragmenting the cDNA using TdT (terminal deoxynucleotidyl transferase); 5) Biotinylating the cDNA fragments using the GeneChip WT Terminal labelling kit (Affymetrix); 6 Repeating steps 1 to 5 with a reference cell line, wherein said reference cell line is MDA-MB-231; 7) Hybridizing 5.5 μg of the biotinylated cDNA fragments obtained in step 5 to a first DNA microarray at 45° C. for 16 hours, and hybridizing 5.5 μg of the biotinylated cDNA fragments obtained in step 6, to a DNA microarray at 45° C. for 16 hours, preferably wherein said microarrays are Affymetrix GeneChip Human Gene 2.0 ST Arrays (Applied Biosystems); 8) Washing and staining the hybridized microarrays, preferably in the Affymetrix GeneChip Fluidics Station 450 (Applied Biosystems); 9) Scanning said stained microarrays with the Affymetrix GeneChip Scanner 3000 7G utilising the Affymetrix GeneChip Command Console® software to produce raw data signal values in the form of CEL files; 10) Normalizing the CEL files to produce gene-level expression values using the implementation of the Robust Multiarray Average (RMA) in the Affymetrix software package (version 1.36.1), preferably as described in R. A. Irizarry et al., Exploration, normalization, and summaries of high density oligonucleotide array probe level data. ..

    Purification:

    Article Title: Deoxy-cytidine or uridine derivatives for use in cancer therapies
    Article Snippet: .. Microarray Method A comprises the following steps: 1) Extracting RNA from cancer/tumour cells of interest, said extraction preferably being performed using a Norgen Total RNA Purification kit (Norgen Biotek Cat nr. 17200); 2) Preparing Poly(A)+ RNA from the extracted RNA, preferably using a MEGApure kit according to the manufacturer's instructions (Ambion); 3) Preparing complementary DNA (cDNA) from the Poly(A)+ RNA by treatment with reverse transcriptase, i.e. performing reverse transcription; 4) Fragmenting the cDNA using TdT (terminal deoxynucleotidyl transferase); 5) Biotinylating the cDNA fragments using the GeneChip WT Terminal labelling kit (Affymetrix); 6 Repeating steps 1 to 5 with a reference cell line, wherein said reference cell line is MDA-MB-231; 7) Hybridizing 5.5 μg of the biotinylated cDNA fragments obtained in step 5 to a first DNA microarray at 45° C. for 16 hours, and hybridizing 5.5 μg of the biotinylated cDNA fragments obtained in step 6, to a DNA microarray at 45° C. for 16 hours, preferably wherein said microarrays are Affymetrix GeneChip Human Gene 2.0 ST Arrays (Applied Biosystems); 8) Washing and staining the hybridized microarrays, preferably in the Affymetrix GeneChip Fluidics Station 450 (Applied Biosystems); 9) Scanning said stained microarrays with the Affymetrix GeneChip Scanner 3000 7G utilising the Affymetrix GeneChip Command Console® software to produce raw data signal values in the form of CEL files; 10) Normalizing the CEL files to produce gene-level expression values using the implementation of the Robust Multiarray Average (RMA) in the Affymetrix software package (version 1.36.1), preferably as described in R. A. Irizarry et al., Exploration, normalization, and summaries of high density oligonucleotide array probe level data. ..

    Multiple Displacement Amplification:

    Article Title: Deoxy-cytidine or uridine derivatives for use in cancer therapies
    Article Snippet: .. Microarray Method A comprises the following steps: 1) Extracting RNA from cancer/tumour cells of interest, said extraction preferably being performed using a Norgen Total RNA Purification kit (Norgen Biotek Cat nr. 17200); 2) Preparing Poly(A)+ RNA from the extracted RNA, preferably using a MEGApure kit according to the manufacturer's instructions (Ambion); 3) Preparing complementary DNA (cDNA) from the Poly(A)+ RNA by treatment with reverse transcriptase, i.e. performing reverse transcription; 4) Fragmenting the cDNA using TdT (terminal deoxynucleotidyl transferase); 5) Biotinylating the cDNA fragments using the GeneChip WT Terminal labelling kit (Affymetrix); 6 Repeating steps 1 to 5 with a reference cell line, wherein said reference cell line is MDA-MB-231; 7) Hybridizing 5.5 μg of the biotinylated cDNA fragments obtained in step 5 to a first DNA microarray at 45° C. for 16 hours, and hybridizing 5.5 μg of the biotinylated cDNA fragments obtained in step 6, to a DNA microarray at 45° C. for 16 hours, preferably wherein said microarrays are Affymetrix GeneChip Human Gene 2.0 ST Arrays (Applied Biosystems); 8) Washing and staining the hybridized microarrays, preferably in the Affymetrix GeneChip Fluidics Station 450 (Applied Biosystems); 9) Scanning said stained microarrays with the Affymetrix GeneChip Scanner 3000 7G utilising the Affymetrix GeneChip Command Console® software to produce raw data signal values in the form of CEL files; 10) Normalizing the CEL files to produce gene-level expression values using the implementation of the Robust Multiarray Average (RMA) in the Affymetrix software package (version 1.36.1), preferably as described in R. A. Irizarry et al., Exploration, normalization, and summaries of high density oligonucleotide array probe level data. ..

    Staining:

    Article Title: Deoxy-cytidine or uridine derivatives for use in cancer therapies
    Article Snippet: .. Microarray Method A comprises the following steps: 1) Extracting RNA from cancer/tumour cells of interest, said extraction preferably being performed using a Norgen Total RNA Purification kit (Norgen Biotek Cat nr. 17200); 2) Preparing Poly(A)+ RNA from the extracted RNA, preferably using a MEGApure kit according to the manufacturer's instructions (Ambion); 3) Preparing complementary DNA (cDNA) from the Poly(A)+ RNA by treatment with reverse transcriptase, i.e. performing reverse transcription; 4) Fragmenting the cDNA using TdT (terminal deoxynucleotidyl transferase); 5) Biotinylating the cDNA fragments using the GeneChip WT Terminal labelling kit (Affymetrix); 6 Repeating steps 1 to 5 with a reference cell line, wherein said reference cell line is MDA-MB-231; 7) Hybridizing 5.5 μg of the biotinylated cDNA fragments obtained in step 5 to a first DNA microarray at 45° C. for 16 hours, and hybridizing 5.5 μg of the biotinylated cDNA fragments obtained in step 6, to a DNA microarray at 45° C. for 16 hours, preferably wherein said microarrays are Affymetrix GeneChip Human Gene 2.0 ST Arrays (Applied Biosystems); 8) Washing and staining the hybridized microarrays, preferably in the Affymetrix GeneChip Fluidics Station 450 (Applied Biosystems); 9) Scanning said stained microarrays with the Affymetrix GeneChip Scanner 3000 7G utilising the Affymetrix GeneChip Command Console® software to produce raw data signal values in the form of CEL files; 10) Normalizing the CEL files to produce gene-level expression values using the implementation of the Robust Multiarray Average (RMA) in the Affymetrix software package (version 1.36.1), preferably as described in R. A. Irizarry et al., Exploration, normalization, and summaries of high density oligonucleotide array probe level data. ..

    Software:

    Article Title: Deoxy-cytidine or uridine derivatives for use in cancer therapies
    Article Snippet: .. Microarray Method A comprises the following steps: 1) Extracting RNA from cancer/tumour cells of interest, said extraction preferably being performed using a Norgen Total RNA Purification kit (Norgen Biotek Cat nr. 17200); 2) Preparing Poly(A)+ RNA from the extracted RNA, preferably using a MEGApure kit according to the manufacturer's instructions (Ambion); 3) Preparing complementary DNA (cDNA) from the Poly(A)+ RNA by treatment with reverse transcriptase, i.e. performing reverse transcription; 4) Fragmenting the cDNA using TdT (terminal deoxynucleotidyl transferase); 5) Biotinylating the cDNA fragments using the GeneChip WT Terminal labelling kit (Affymetrix); 6 Repeating steps 1 to 5 with a reference cell line, wherein said reference cell line is MDA-MB-231; 7) Hybridizing 5.5 μg of the biotinylated cDNA fragments obtained in step 5 to a first DNA microarray at 45° C. for 16 hours, and hybridizing 5.5 μg of the biotinylated cDNA fragments obtained in step 6, to a DNA microarray at 45° C. for 16 hours, preferably wherein said microarrays are Affymetrix GeneChip Human Gene 2.0 ST Arrays (Applied Biosystems); 8) Washing and staining the hybridized microarrays, preferably in the Affymetrix GeneChip Fluidics Station 450 (Applied Biosystems); 9) Scanning said stained microarrays with the Affymetrix GeneChip Scanner 3000 7G utilising the Affymetrix GeneChip Command Console® software to produce raw data signal values in the form of CEL files; 10) Normalizing the CEL files to produce gene-level expression values using the implementation of the Robust Multiarray Average (RMA) in the Affymetrix software package (version 1.36.1), preferably as described in R. A. Irizarry et al., Exploration, normalization, and summaries of high density oligonucleotide array probe level data. ..

    Variant Assay:

    Article Title: Enhanced Risk Prediction for Coronary Heart Disease by Leveraging Polygenic Risk Score and Clinical Risk Score in European Hypertensive Adults.
    Article Snippet: .. SNP genotype data of all participants were acquired from the Affymetrix 6.0 DNA microarray platform (Affymetrix, Santa Clara, CA, USA) and analyzed using the Birdseed variant-calling algorithm. ..



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    Image Search Results


    Gene Expression in the Pituitary Gland of Anxa5 -/- Mice

    Journal: Journal of the Endocrine Society

    Article Title: Augmentation of Nr4a3 and Suppression of Fshb Expression in the Pituitary Gland of Female Annexin A5 Null Mouse

    doi: 10.1210/jendso/bvaa096

    Figure Lengend Snippet: Gene Expression in the Pituitary Gland of Anxa5 -/- Mice

    Article Snippet: Gene chip analysis and cDNA microarray data were carried out at GeneticLab Co., Ltd. (Sapporo, Japan).

    Techniques: Gene Expression, Membrane, Binding Assay

    VERIFICATION OF GENE ARRAY RESULTS BY REAL-TIME QUANTITATIVE PCR ANALYSIS

    Journal: Gene Expression

    Article Title: Distinct Gene Expression Profile of Human Mesenchymal Stem Cells in Comparison to Skin Fibroblasts Employing cDNA Microarray Analysis of 9600 Genes

    doi:

    Figure Lengend Snippet: VERIFICATION OF GENE ARRAY RESULTS BY REAL-TIME QUANTITATIVE PCR ANALYSIS

    Article Snippet: Bone sialoprotein is known as initiator of matrix mineralization and is upregulated during the mineralization phase of osteoblast differentiation ( 27 ). cDNA Microarray We employed a custom-made cDNA microarry chip that was constructed at the Institute for Molecular Biology and Tumor Research Marburg as described previously ( 8 ).

    Techniques: Real-time Polymerase Chain Reaction, Gene Expression, Binding Assay